Experimental protocols for DMD animal models

Experimental protocols for DMD animal models

The availability of standardized operating procedures (SOPs) to unify experimental protocols used to test the effects of new treatments in animal models is a step that will undoubtedly improve the comparability of studies from different laboratories. SOPs are not meant to be mandatory but are designed to be a point of reference and should not prevent innovation and further improvement of the existing protocols. To ensure reproducibility and quality, each SOP has been drawn up by a group of independent researchers (listed in each protocol) and is updated on a regular basis.

Two meetings were hosted with specialists from all over the world to discuss and create this collection of SOPs, in full collaboration with the Senator Paul D. Wellstone Muscular Dystrophy Cooperative Research Center at Children's National Medical Center in Washington DC and with the US National Institutes of Health (NIH)-Wellstone Muscular Dystrophy Cooperative Network, and with the generous support of the Foundation to Eradicate Duchenne Inc, the US National Institutes of Health and TREAT-NMD. These workshops were described in a meeting report published in Neuromuscular Disorders and available here.

The SOPs that have already been completed can be downloaded from this site and others will be added in the future. The last date each SOP was reviewed can be found under the code on the left of the page.

Mdx Model

31 July 2008

The recovery score: a common, quantitative and comparative scoring system

Jean-Marie Gillis

28 January 2014

Quantitative determination of muscle fibre diameter (minimal Feret’s diameter) and percentage of centralized nuclei

Michael Erb

5 June 2015

Measuring isometric force of isolated mouse muscles in vitro

Elisabeth R. Barton

28 June 2016

Use of patch-clamp technique to study voltage-independent cationic channels in normal and dystrophic skeletal muscle fibers

Philippe Gailly

30 January 2014

Determination of hydroxyproline content as a measure of fibrosis

C. George Carlson

25 January 2014

Quantification of histopathology in Haemotoxylin and Eosin stained muscle sections

Miranda Grounds

27 March 2014

Use of treadmill and wheel exercise for impact on mdx mice phenotype

Annamaria De Luca

12 July 2017

Behavioural and Locomotor Measurements Using Open Field Animal Activity Monitoring System

Kanneboyina Nagaraju

23 May 2015

Use of treadmill and wheel exercise to assess dystrophic state

Robert W. Grange

29 June 2016

The use of hanging wire tests to monitor muscle strength and condition over time Maaike van Putten

29 June 2016

The use of four limb hanging tests to monitor
muscle strength and condition over time
C. George Carlson

11 March 2014

Use of grip strength meter to assess the limb strength of mdx mice

Annamaria De Luca

12 July 2016

Respiratory system evaluation

Tejvir S. Khurana

4 June 2015

Non-invasive echocardiographic assessment of cardiac function in the mdx mouse model

Christopher Spurney

10 January 2013

Left ventricular pressure-volume (PV) loops in mdx

Paul Herijgers

30 June 2016

Measuring isometric force of isolated mouse skeletal muscles in situ

Gordon Lynch

1 November 2010

Whole body tension measurements

C. George Carlson

Mouse Models of DMD

25 March 2014

Serum Creatine Kinase analysis in mouse models of muscular dystrophy

Kanneboyina Nagaraju

GRMD Model

13 June 2015

Measuring tetanic isometric force at the tibiotarsal joint in vivo in GRMD dogs

Joe Kornegay

13 June 2015

Measuring isometric force decrement after eccentric contraction in vivo in GRMD dogs

Joe Kornegay

13 June 2015

Measuring tibiotarsal joint angle to assess contractures in vivo in GRMD dogs.

Joe Kornegay

13 June 2015

Evaluation of Muscles by Magnetic Resonance Imaging (MRI)

Joe Kornegay

Cardiac Protocols for Duchenne Animal Models

Parent Project Muscular Dystrophy (PPMD) and the National Heart, Lung, and Blood Institute (NHLBI) convened a Working Group meeting to address the current issues relevant to Duchenne cardiomyopathy. A critical knowledge gap identified by The Working Group is the insufficiency of current standard operating procedures (SOPs) for preclinical animal studies. A task force committee was organized to refine and update cardiac SOPs for the assessment of cardiac disease in rodent and large animal models of DMD. This protocol repository contains detailed descriptive methodologies from leading laboratories in the field. The repository is managed by PPMD and will be updated regularly by the task force committee.

These cardiac SOPs can be downloaded from the PPMD web site

Stay up to date with our operating procedures

Stay up to date with our SOPs

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Disclaimer: TREAT-NMD and the authors of the SOPs disclaim, without limitation, all liability for any loss or damage of any kind, including any direct, indirect or consequential damages, which might be incurred in connection with the information and procedures contained in these SOPs.

14 Jun 2018