Experimental protocols for DMD animal models

Experimental protocols for DMD animal models
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  • Raffaella Willmann
    Raffaella Willmann
    Raffaella Willmann studied Biology in Italy and obtained her PhD in Biochemistry in Konstanz (Germany)....

The availability of standardized operating procedures (SOPs) to unify experimental protocols used to test the effects of new treatments in animal models is a step that will undoubtedly improve the comparability of studies from different laboratories. SOPs are not meant to be mandatory but are designed to be a point of reference and should not prevent innovation and further improvement of the existing protocols. To ensure reproducibility and quality, each SOP has been drawn up by a group of independent researchers (listed in each protocol) and is updated on a regular basis.

Two meetings were hosted with specialists from all over the world to discuss and create this collection of SOPs, in full collaboration with the Senator Paul D. Wellstone Muscular Dystrophy Cooperative Research Center at Children's National Medical Center in Washington DC and with the US National Institutes of Health (NIH)-Wellstone Muscular Dystrophy Cooperative Network, and with the generous support of the Foundation to Eradicate Duchenne Inc, the US National Institutes of Health and TREAT-NMD. These workshops were described in a meeting report published in Neuromuscular Disorders and available here.

The SOPs that have already been completed can be downloaded from this site and others will be added in the future. The last date each SOP was reviewed can be found under the code on the left of the page.

Mdx Model


DMD_M.1.1.001
31 July 2008

The recovery score: a common, quantitative and comparative scoring system

Jean-Marie Gillis

DMD_M.1.2.001
28 January 2014

Quantitative determination of muscle fibre diameter (minimal Feret’s diameter) and percentage of centralized nuclei

Michael Erb

DMD_M.1.2.002
9 March 2013

Measuring isometric force of isolated mouse muscles in vitro

Elisabeth R. Barton

DMD_M.1.2.005
6 February 2014

Use of patch-clamp technique to study voltage-independent cationic channels in normal and dystrophic skeletal muscle fibers

Philippe Gailly

DMD_M.1.2.006
30 January 2014

Determination of hydroxyproline content as a measure of fibrosis

C. George Carlson

DMD_M.1.2.007
25 January 2014

Quantification of histopathology in Haemotoxylin and Eosin stained muscle sections

Miranda Grounds

DMD_M.2.1.001
27 March 2014

Use of treadmill and wheel exercise for impact on mdx mice phenotype

Annamaria De Luca

DMD_M.2.1.002
30 January 2014

Behavioural and Locomotor Measurements Using Open Field Animal Activity Monitoring System

Kanneboyina Nagaraju

DMD_M.2.1.003
24 January 2013

Use of treadmill and wheel exercise to assess dystrophic state

Robert W. Grange

DMD_M.2.1.004
15 January 2014

The use of hanging wire tests to monitor muscle strength and condition over time Maaike van Putten

DMD_M.2.1.005
15 January 2014

The use of four limb hanging tests to monitor
muscle strength and condition over time
C. George Carlson

DMD_M.2.2.001
11 March 2014

Use of grip strength meter to assess the limb strength of mdx mice

Annamaria De Luca

DMD_M.2.2.002
22 January 2014

Respiratory system evaluation

Tejvir S. Khurana

DMD_M.2.2.003
29 January 2013

Non-invasive echocardiographic assessment of cardiac function in the mdx mouse model

Christopher Spurney

DMD_M.2.2.004
10 January 2013

Left ventricular pressure-volume (PV) loops in mdx

Paul Herijgers

DMD_M.2.2.005
24 January 2014

Measuring isometric force of isolated mouse skeletal muscles in situ

Gordon Lynch

DMD_M.2.2.006
1 November 2010

Whole body tension measurements

C. George Carlson

Mouse Models of DMD

MD_M.2.2.001
25 March 2014

Serum Creatine Kinase analysis in mouse models of muscular dystrophy

Kanneboyina Nagaraju

GRMD Model


DMD_D.2.2.001
25 January 2013

Measuring tetanic isometric force at the tibiotarsal joint in vivo in GRMD dogs

Joe Kornegay

DMD_D.2.2.002
31 January 2013

Measuring isometric force decrement after eccentric contraction in vivo in GRMD dogs

Joe Kornegay

DMD_D.2.2.003
24 January 2013

Measuring tibiotarsal joint angle to assess contractures in vivo in GRMD dogs.

Joe Kornegay

DMD_D.2.2.004
8 February 2013

Evaluation of Muscles by Magnetic Resonance Imaging (MRI)

Joe Kornegay

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Disclaimer: TREAT-NMD and the authors of the SOPs disclaim, without limitation, all liability for any loss or damage of any kind, including any direct, indirect or consequential damages, which might be incurred in connection with the information and procedures contained in these SOPs.

 
09 Apr 2014