Experimental protocols for SMA animal models

Experimental protocols for SMA animal models

The availability of standardized operating procedures (SOPs) to unify experimental protocols used to test the effects of new treatments in animal models is a step that will undoubtedly improve the comparability of studies from different laboratories. SOPs are not meant to be mandatory but are designed to be a point of reference and should not prevent innovation and further improvement of the existing protocols. To ensure reproducibility and quality, each SOP has been drawn up by a group of independent researchers (listed in each protocol) and is updated on a regular basis.

TREAT-NMD supported the workshop dedicated to the creation of a collection of SOPs for SMA animal models, where 30 key researches from Europe and North America presented and discussed the parameters most commonly used to assess drug efficacy in SMA mouse models.

The workshop report is now available to download from this page.

The SOPs that have already been completed can be downloaded from this site and others will be added in the future. The last date each SOP was reviewed can be found under the code on the left of the page.

SMA mouse model

SMA_M.1.2.001
11 July 2016

Synaptic transmission alterations in SMA mice / Electrophysiology

Lucia Tabares

SMA_M.1.2.002
21 June 2016

Muscle Function evaluation through isometric force measurements in mouse models for muscular atrophy

Chien-Ping Ko

SMA_M.1.2.003
5 June 2015

Imaging neuromuscular junction pathology in mouse models of SMA

Tom Gillingwater

SMA_M.1.2.004
11 July 2017

Cell Body-Motor Neuron counts

Ke Ning

SMA_M.1.2.005
5 June 2016

ELISA to quantify SMN protein levels

Brunhilde Wirth

SMA_M.1.2.006
5 June 2016

Semiquantitative Western Blot

Brunhilde Wirth

SMA_M.1.2.007
8 July 2016

Human and murine SMN transcript analysis by absolute real time PCR

F. Danilo Tiziano

SMA_M.2.1.001
6 June 2016

Use of pen test (balance beam) to assess motor balance and coordination in mice

Rashmi Kothary

SMA_M.2.1.002
11 July 2017

Grip strength

Stefania P. Corti

SMA_M.2.2.001
14 March 2011

Behavioral Phenotyping for Neonates: Hind Limb Suspension Test (a.k.a. Tube Test)

Bassem El-Khodor
SMA_M.2.2.002
23 June 2016

Compound Muscle Activated Potential (CMAP) recordings from the triceps surae

Veronica Setola
SMA_M.2.2.003
4 June 2015

Influence of housing, feeding, and handling conditions on SMA mouse performance

Charlotte Sumner
& Arthur Burghes

SMA_M.2.2.004
10 October 2011

Genotyping protocols for commonly used SMA model mice

Christine DiDonato

MD_M.2.2.002
8 April 2014

Behavioral Phenotyping for neonates: Righting reflex

C. DiDonato &
L. Bogdanik

Stay up to date with our operating procedures

Stay up to date with our SOPs

If you wish to be informed when new operating procedures become available on this website please leave your name and email address below.

Please be aware that we will not pass your details on to anyone else. We will only use the information you submit here for its original intended purpose as stated above.

If you wish to be removed from the list please contact michael.hails@ncl.ac.uk who will be happy to help.

Disclaimer: TREAT-NMD and the authors of the SOPs disclaim, without limitation, all liability for any loss or damage of any kind, including any direct, indirect or consequential damages, which might be incurred in connection with the information and procedures contained in these SOP

 
15 Aug 2017